BrainXell’s neuronal products are widely used in high-content analysis (HCA), high-throughput screening, and large-scale automated live-cell imaging. HCA uses multi-parameter image processing and visualization tools to extract quantitative data from cell populations such as spatial distribution of proteins or cell structures.
HCA has been employed for screening of environmental toxins using iPSC-derived motor neurons that were gene-edited to ubiquitously express eGFP (Catalog number BX-0101). Following optimization of culture conditions in a 384-well format and imaging parameters for neuron number and neurite length, high-content imaging yielded a sensitive and robust system with a Z-prime value greater than 0.5.
The detection of neurons and neurites are demonstrated here. Original images (A) were processed to detect cell bodies (B) and neurites (C) for the control (DMSO) and 20 µM rotenone conditions. Dose-response curves for both neuron number (E) and total neurite length per well (F) were measured.
