Pluripotent Stem Cells on Demand
BrainXell provides rapid, low-cost, footprint-free, xeno-free derivation of induced pluripotent stem cells (iPSCs) from human fibroblasts on a fee-for-service basis. We are specialists, committed to producing iPSCs in the most efficient manner possible. As a result of our focused approach, we can bring you the following key benefits:
- Technology
- Our approach leverages an advanced form of mRNA reprogramming, which stands out as the fastest and most efficient method for converting fibroblasts into pluripotent stem cells. Unlike traditional techniques that rely on viral vectors or DNA-based reprogramming, our method uses synthetic, highly optimized mRNA molecules to transiently express key reprogramming factors. This not only enhances efficiency but also eliminates the risk of genomic integration, ensuring the resulting induced pluripotent stem cells (iPSCs) maintain a pristine genetic profile. By fine-tuning the stability and delivery of mRNA, we achieve rapid, reproducible reprogramming with high yield and minimal cellular stress, making this technique a powerful tool for regenerative medicine, disease modeling, and drug discovery.
- Timeline
- Overall turnaround is typically 3 months, from the time we receive fibroblasts to delivery of iPSCs. This includes the time required to bring batches of lines into culture and the fact that a minority of lines require extra passages before being release-ready. We will keep you posted and offer staged delivery options when a few problematic lines could impact the overall project schedule. Turnaround can also be affected by the number of lines you submit at once, and by current workload. Work queue priority is set by the date of purchase order receipt.
- Deliverables
- We supply both clonal and pooled iPSC lines reprogrammed from fibroblasts. Each iPSC line is delivered with a COA and thaw report presenting phase contrast and immunostaining images demonstrating iPSC morphology and generalized expression of canonical molecular markers (OCT4/NANOG). All iPSC lines are tested for mycoplasma contamination, and identity by STR analysis. Additional characterization options include karyotyping, fibroblast STR and alternate marker expression is available upon request.
Custom Differentiation Assay Services
Your Project, Your Scale
Whether you require multiple lines produced in small batches or a billion neurons from a single cell line, we understand that aims and goals can differ. That’s why we provide tailored services to adjust cell line numbers and batch quantities according to your individual needs. Additionally, we offer storage for your specific cell lines, enabling quick production of extra or recurring batches as necessary.
Upon receipt of your iPSC cells, we can typically deliver up to half a billion differentiated neurons in four to six months.