BrainXell Sensory Neurons
Use / Functionality: Human iPSC-derived sensory neurons validated for key sensory biology markers and functional readouts, enabling robust studies in pain biology, sensory transduction, and ion channel pharmacology.
Reproducibility / Scalability: Demonstrated strong lot-to-lot consistency across production runs, with stable qPCR Cq values for key sensory neuron markers relative to GAPDH, supporting reliable experimental outcomes and scalable screening and discovery workflows.
Expression Profile: Robust expression of hallmark sensory neuron genes and proteins, including Nav1.7–1.9 sodium channels, TRPV1, TRPM8, P2RX3, PRPH, ISL1, and POU4F1, with opioid receptor expression (OPRM1, OPRK1, OPRL1). Immunocytochemistry confirms sustained protein-level expression of Brn3a, Peripherin, and Islet 1 through four weeks in culture, consistent with transcriptomic data.
The majority of BrainXell’s items and sizes are available for immediate shipment. Please inquire regarding your order to confirm the current inventory.
| Catalog Number | Cell Type | IPSC Line | Sex | Reporter | Edit | 1M Size Vial | 2.5M Size Vial | 5M Size Vial | hf:categories | hf:att:pa_ipsc-line | hf:att:pa_sex | hf:att:pa_reporter | hf:att:pa_edit |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BX-1200-32 | Sensory Neurons | WC-32 | F | $650.00 Add to cart | $1,060.00 Add to cart | $1,750.00 Add to cart | sensory-neurons neurons | wc-32 | f | ||||
| BX-1200-30 | Sensory Neurons | WC-30 | M | $650.00 Add to cart | $1,060.00 Add to cart | $1,750.00 Add to cart | sensory-neurons neurons | wc-30 | m |
Overview
| Product ID | BX-1200-30 |
|---|---|
| Description | BrainXell Sensory Neurons are human iPSC-derived peripheral sensory neurons engineered for robust sensory biology and functional pharmacology applications. These neurons express key nociceptive and thermosensory markers and demonstrate reproducible maturation, lot-to-lot consistency, and functional responses to well-characterized agonists and inhibitors. Sensory neurons are suitable for endpoint assays beginning three weeks post-thaw and continue to mature through four weeks in culture. |
Maturation
- Post-thaw neuronal maturation over 3–4 weeks
- Network formation observed by phase-contrast imaging
- Suitable for endpoint and functional assays after ~21 days
Reproducibility
BrainXell Sensory Neurons show strong lot-to-lot reproducibility across multiple production runs. Quantitative PCR analysis demonstrates consistent Cq values for key sensory neuron markers relative to the housekeeping gene GAPDH, with each data point representing an independent lot. This reproducibility supports reliable experimental outcomes and scalable use across screening and discovery workflows.
BrainXell sensory neurons express key sensory-neuron markers including voltage gated sodium channels (Nav1.7, Nav1.8, Nav1.9), TRP channels (TRPV1, TRPM8), and others and show lot-to-lot consistency.
Expression
Sensory neurons abundantly express hallmark sensory neuron genes and proteins, including voltage-gated sodium channels (Nav1.7, Nav1.8, Nav1.9), sensory transduction markers (TRPV1, TRPM8, P2RX3), and sensory neuron-associated genes such as PRPH, ISL1, and POU4F1. Opioid receptors (OPRM1, OPRK1, OPRL1) are also expressed. Immunocytochemistry confirms protein-level expression of markers including Brn3a, Peripherin, and Islet 1 throughout a four-week culture period, consistent with transcriptomic data.
BrainXell sensory neurons express key sensory neuron markers throughout a 4-week culture period, consistent with observed mRNA expression levels.
Functionality
BrainXell Sensory Neurons are functionally validated for sensory transduction and pharmacological studies. Neurons respond to canonical agonists including ATP (P2RX3), capsaicin (TRPV1), icilin (TRPM8), histamine, and noxious heat, with responses detected as early as Day 16 post-thaw. Functional specificity is demonstrated through pharmacological blockade, including TRPV1 antagonism with capsazepine and selective inhibition of Nav1.7 and NaV1.8 channels. These cells are well suited for pain research, target validation, ion channel pharmacology, and sensory biology studies.
BrainXell sensory neurons respond to P2RX3, TRPV1 and TRPM8 agonists
BrainXell sensory neurons response to Capsaicin is blocked by TRPV1 antagonist
BrainXell sensory neurons respond to Capsaicin and Histamine as early as 16 days post-thaw
BrainXell sensory neurons respond to ATP and noxious heat
BrainXell sensory neurons respond to Nav1.7 and Nav1.8 inhibitors
Whole-cell patch-clamp recordings demonstrate a robust, dose-dependent response of BrainXell sensory neurons to capsaicin
Protocols
BrainXell Sensory Neurons are supplied cryopreserved and ready for straightforward thaw, plating, and maintenance using BrainXell-recommended media and protocols. Cells are compatible with calcium imaging, multi-electrode array (MEA) recordings, and endpoint molecular assays. Detailed protocols for thawing, culture, maturation timing, and functional assays are available upon request to support seamless integration into existing workflows.
Build more physiologically relevant sensory biology models by pairing BrainXell Sensory Neurons with complementary human neuronal and glial cell types. Co-culture systems incorporating spinal astrocytes and microglia support studies of neuroinflammation, neuron–glia signaling, and functional responses relevant to pain biology and translational neuroscience. Additional BrainXell offerings, including disease models and custom assay services, enable seamless progression from discovery through validation.
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