Spinal Astrocytes

Origin: Human iPSC line

2M viable cells/vial

Contents: 1 vial of cells and corresponding BrainXell supplement

Cryopreserved

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SPECIFICATIONS

Marker Expression and Morphology:

Spinal Astrocytes (BX-0650) express key astrocyte markers GFAP (green) and S100β (red) at >90% and exhibit mature process-bearing morphologies (shown here with a DAPI stain).

spinal astrocyte GFAP black with lots of green and few purple

Labelling by the spinal-specific marker HOXB4 (red) with a Hoescht stain highlights the regional specificity of the Spinal Astrocytes.

Microscope image of Spinal Astrocyte HOXB4

Function: Spinal Astrocytes (BX-0650) promote mature motor neuron activity. Co-culturing motor neurons with spinal astrocytes promotes maximal calcium influx within 10 days.

Co-culture spinal astrocytes with motor neurons also accelerates the development of activity recorded by multi-electrode array.

PROTOCOLS

Recommended protocols developed by BrainXell utilize proprietary supplements that are included with each purchase of cells. The recommended protocol will depend on the application. Contact BrainXell application scientists to learn more.

Culturing Astrocytes
Co-Culture of Neurons and Astrocytes

Read More About BrainXell’s Spinal Astrocytes:
Generation of Mature Cortical and Spinal Astrocytes from Human iPSCs